Zhuravlev Yu.N., Pisetskaya N.F., Muzarok T.I., Shumilova L.A., Reifman V.G.
В журнале Virology
Год: 1975 Том: 64 Номер: 1 Страницы: 43-48
14C-labeled TMV was used to examine virus attachment to tobacco protoplasts. About 600 virus particles per protoplast were attached under experimental conditions (1 μg/ml of TMV, 1 μg/ml of poly-L-ornithine, 15 × 105 cells/ml of protoplasts, 10 min at 25°). The number of virus particles associated with protoplasts increases during the course of inoculation, 10-15 min exposures being optimal. Approximately linear enhancement of attachment was observed at 1-1,500 μg/ml TMV-concentration interval. Poly-l-ornithine (1 μg/ml) was shown to be a potent stimulator of virus retention at all TMV concentrations used. Increased poly-l-ornithine concentration results in greater virus retention. The authors conclude that virus attachment per se is stimulated by poly-l-ornithine and maintain that the processes of attachment and penetration should be considered more specifically. © 1975.
