Laser Micromechanical Sensor for Measurement of the Mass of Bacterial Cell Colonies

Efimov T.A., Degtyarenko A.I., Romashko R.V., Shkryl Y.N., Bezruk M.N., Chernev I.M., Galkin K.N., Storozhenko D.V.

В журнале Bulletin of the Russian Academy of Sciences: Physics

Год: 2024 Том: 88 Выпуск: Suppl 3 Страницы: 322–324

We presented results of applying an adaptive interferometry technique, based on dynamic holograms recorded in photorefractive crystal, to accurately determine the mass of biofilm bacteria colonies by the measuring of microcantilever frequency shift. During the experimental testing, biofilms of Escherichia coli were grown on the surface of a cantilever previously coated with a BaF2 film. The proposed experimental setup is completely non-contact due to using an optical method of excitation and detection of cantilever oscillations. Based on the measured shift in the resonant frequency, the mass of the biofilm grown on the cantilever was calculated to be (2.072 ± 0.003) × 10–9 gram.

DOI 10.1134/S1062873824709759

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