RNA inhibition of the JAZ9 gene increases the production of resveratrol in grape cell cultures

Makhazen D. S., Veremeichik G. N., Shkryl Y. N., Grigorchuk V. P., Tchernoded G. K., Degtyarenko A. I., Bulgakov V. P.

В журнале Plant Cell, Tissue and Organ Culture

Год: 2021

In the human diet, plants of the genus Vitis are considered the main source of resveratrol, which possesses promising pharmacological activities. In plants, resveratrol plays an important role in the response to biotic and abiotic stress, and is a classic phytoalexin due to its antimicrobial properties. Jasmonic acid (JA) signaling is important for the regulation of resveratrol biosynthesis, in which JA/jasmonoyl-L-isoleucine-regulated JAZ proteins are important players. Using Vitis vinifera cell cultures, we investigated the role of the VvJAZ9 gene in resveratrol accumulation. Our results showed that inhibition of the VvJAZ9 gene (the closest homolog of Arabidopsis thaliana AtJAZ1) by artificial micro-RNA technology resulted in a 10-fold increase in resveratrol content from 0.2 to 2 mg/g DW. Despite a slight decrease in the growth of transgenic cells, the productivity of the transgenic cell line was 1.38 mg/L, which was almost 7 times higher than in the wild-type culture. Our results provide evidence that silencing of JAZ genes may be promising for producing plants or plant cell cultures with increased resveratrol levels. The advantage of the proposed technology is that inhibition of JAZ genes could reproduce the effects of exogenously added JA and reduce the toxic effect of jasmonates on the growth of cell cultures.

DOI 10.1007/s11240-021-02152-w